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Methodology of Study in Aerobiology
Types of aerobiological traps
The capture of airborne particles in the atmosphere can be done basically by two methods. Gravimetric and Volumdetric methods.

Gravimetric methods capture particles passively, simply dropping them to the ground. Particles are collected on adhesive surfaces or culture media. This methodology is followed among other methods by Durham and Cour traps.

Volumetric methods involve air suction actively and the consequent control of flow, so, it is possible to know the volume of air sampled. This medology is followed by Andersen or Hirst traps.

Spore traps used by the research team are Hirst type
We have chosen Hirst type spore traps in the network of spore traps as it allows continuous recording of the atmosphere with a high degree of efficiency. These traps draw air at a rate similar to that of human breath (10 liters per minute). The suctioned air impinges on a transparent plastic tape (melinex) covered by an adhesive substance of great stability to different environmental conditions (white petrolatum). The tape is mounted on a rotating drum with a mechanical clockwork giving a full turn in a week. The film once exposed cut into seven pieces that are mounted on slides that are covered with glycero-gelatin fuschina stained on the coverslip.

Particle counting using the light microscope
Each microscope slide contains particles collected during a period of 24 hours. To separate time intervals a piece striped acetate at intervals of 2 mm is used, this is is placed in the rear of the preparation. Four or two longitudinal scans are performed in the center of the slide, always separated one millimeter each other. Pollen grains observed are recorded with an objective of 40 magnification. Data from the scans are summed and converted to values ​​of grains per cubic meter by multiplying by the appropriate factor.

Description of the research line
Continuous recording of airborne particles (grains pollen and spores), preventive applications allergies or hay fever, relationship environmental parameters, study of the temporal and spatial distribution, analysis of the causes of their variations.

UNESCO codes
241703 Plant Biology, General Botany
320701 Medicine Sciences, Pathology, Allergy
250203 Bioclimatology
241603 Paleontology, Palynology

Papers appear in scientific publications
Aerobiologia, Grana, Allergy, International, Journal of Biometeorology, Annals of Botany, European Journal of Phycology, Allergology Et Immunopathology, Revista Española de Alergología e Inmunología, Clínica, Boletín de Sanidad Vegetal-Plagas, Rev. Port. Imunoalergol., Acta Botánica Malacitana, Red Española de Aerobiología, Polen.